Poot-Slap

Poot-Slap is the most well known song by 3-Dee, originally named 2 Copies Sealed, a hip hop "booty rap" group comprised of members Nehi, Yoo-Hoo, Sundrop, M-Chaps, 4xMike. You may know the last two members better as Matt and Mike Chapman ("The Brothers Chaps"), the creators of Homestar Runner. These songs were made when they were in high school and/or college. 3-Dee's music can occasionally be heard/referenced in various Homestar cartoons; Poot-Slap in particular has been used in The Luau, impression, and the Luau & Strong Bad main pages. The track is also available for download from the Downloads page.

It's worth noting though that there are three official versions:
1: The version heard on the website
2: A much more vulgar extended version from Thorax Corporation, as heard on the 3-Dee Booty album
3: A low-quality remix version mashed up with the musical accompaniment of "Smoke On The Water". This was once linked on the oldest downloads menu, but is now a secret page.